Next Generation Sequencing (NGS) is a general name for high throughput DNA sequencing technologies, which intend to lower the cost and higher the speed of DNA sequencing beyond what is possible with standard dye-terminator methods.
|Method||Pyrosequencing (454)||Sequencing by synthesis (Illumina)||Chain termination (Sanger sequencing)|
|Read length||700 bp||50 to 250 bp||400 to 900 bp|
|Reads per run||1 million||Upto 3 billion||N/A|
|Time per run||24 hours||1 to 10 days, depending upon sequencer and specified read length||20 minutes to 3 hours|
|Cost per 1 million bases (in US $)||10$||$0.05 to $0.15||$2400|
|Advantages||Long read size. Fast||Potential for high sequence yield, depending upon sequencer model and desired application.||Long individual reads. Useful for many applications|
|Disadvantages||Runs are expensive. Homopolymer errors.||Equipment can be very expensive.||More expensive and impractical for larger sequencing projects.|